In vivo multi-antigen targeting with iDuo NK cells. (A) Schematic for the experiments to test multi-antigen targeting with iDuo NK cells. NSG mice were injected IV with 1 × 105 Raji cells stably expressing firefly luciferase. After allowing the tumors to engraft for 1 day, mice were either left untreated (n = 8), received three IV injections of rituximab (3 μg) alone (n = 8), 1 × 107 iDuo NK cells alone (n = 8), or 1 × 107 iDuo NK cells along with rituximab (n = 8). Rituximab and cell doses were given on days 1, 4, and 7 post-tumor engraftments. (B) Bioluminescence images through the first 5 weeks of the experiments. (C) Graphical representation of cumulative BLI data. Statistical significance was determined by two-way ANOVA. Shown is mean ± standard deviation. (D) Kaplan–Meier curves for the in vivo Raji experiment. Statistical significance was determined using a log-rank (Mantel–Cox) test. ∗P ≤ .05, ∗∗P ≤ .01, ∗∗∗P ≤ .001, ∗∗∗∗P ≤ .0001.
