T-cell exhausted profiles are observed in patients treated with azithromycin (AZM). (A) Heatmap representing scaled expression of 14 functional antigens used to identify 25 functional state clusters with the use of FlowSOM algorithm among 30 T-cell phenotypic subsets. Below, dot plot showing percentage of functional state clusters in each T-cell subset. Only functional state clusters with >4% of the corresponding cell subsets are drawn. Letters indicate a significant difference between AZM and placebo (PLA) groups and the corresponding panel. (B-F) Boxplots of statistically different state subsets between AZM and PLA cohorts. For visualization purposes, square root transformation was applied on the y-axis. All P values were calculated by means of 2-sided Wilcoxon signed rank test.