Murine MYCN-driven T-cell lymphoma recapitulates human PTCL with a high MYC signature. (A) Heat map showing the top differentially expressed genes in MYCN-driven TCL compared with normal CD4 T cells and depicting the transcription factor binding motifs of the top transcription factors identified by i-CisTarget in the up- and downregulated genes. NES = normalized enrichment score. (B) NESs for different hallmark gene sets in MYCN-driven TCL compared with normal CD4 T cells. (C) GSEA showing enrichment of an embryonic stem cell–like signature as described by Wong et al.²⁵ (D) Centered read-density heat maps showing binding locations of MYCN, H3K4me3, H3K27ac, and H3K27me3 in MYCN-driven TCL. Heat maps centered and ranked on MYCN signal strength. (E) ChIP-seq peak occurrence of MYCN in relation to promoter regions (H3K4me3⁺) and enhancer regions (H3K27ac⁺ and H3K4me3⁻). (F) Venn diagram showing the total amount of ChIP-seq peaks of MYCN in promoters of genes that are differentially (Diff.) expressed in MYCN-driven TCL compared with normal CD4 T cells. (G) Volcano plot showing the log2 fold change of direct MYCN target genes in MYCN-driven TCL compared with CD4 T cells. (H) GSEA to show negative enrichment of the top 500 downregulated direct MYCN target genes and positive enrichment of the top 500 upregulated direct MYCN target genes in the differentially expressed genes in PTCL with a high vs low MYC signature. See also supplemental Figure 3.