Imaging of the clots obtained after SP treatment of VWF-KO mice. (A) Representative images of the fluorescent staining of clots recovered after amputation of the tail tip of mice with VWF WT and VWF-KO injected with SP (2 mg/kg). Dashes represent the vessel’s edges. Rhodamine-labeled SP are visible in red, fibrin/fibrinogen is visible in magenta and CD41-labeled platelets appear in green. (B) Quantification of the platelets (CD41⁺) and fibrin area and fluorescence intensity in WT (n = 3) and VWF-KO mice injected with SP (n = 3). Data are presented as mean ± SD. Statistical analysis was performed using a Mann-Whitney test. (C) Scanning electron microscopy of the clot recovered after tail vein transection in mouse with VWF-KO left untreated and treated with SP. In the upper images, black arrows indicate the transection area recognizable by the hair that appear sectioned and the blue arrows point to the clot. In the bottom left image, red arrows show individualized red blood cells. In the bottom right image, blue arrow shows the clot characterized by aggregated red blood cells and red arrows show individualized red blood cells. Two mice per group were imaged and representative images are shown.