Inhibition of integrin αIIbβ3 activation reduces thrombus formation in TTP. WT mice were injected with antibodies to ADAMTS13 and human plasma VWF to induce TTP. At 24 hours after TTP induction, blood was collected to analyze platelet count, and lungs were collected to analyze thrombus formation. Saline control or eptifibatide (15 mg/kg, body weight) was injected when VWF was administrated and then was given every 6 hours after the first administration. (A) Representative immunofluorescence images of thrombi in lungs of saline- and eptifibatide-treated mice with TTP. (B) Quantification of thrombus formation in lung shown in (A). Quantification of the pixels of CD41-positive areas in each field of images. (C) Platelet counts in mice with TTP. Platelet counts in mice at 7 days before experiments (basal level) and at 24 hours after induction of TTP were quantified. Each dot represented a datum from 1 mouse. The data are representative of 5 independent experiments, and data represent mean ± SD. *P < .05.