TIM-3 expression on T cells, T-regs, and NK cells in patients with CML and in healthy donors. (A) TIM-3 expression as a proportion of T cells was significantly higher in patients who experienced MolR compared with those who experienced TFR. Healthy donors (HD) had significantly lower TIM-3 expression compared with both patients in MolR and TFR groups. (B) Absolute number of TIM-3⁺ T cells was higher in the patients in MolR group than those in the TFR group (C) TIM-3 expression as a proportion of CD4⁺T cells was higher in MolR group than in TFR group and the expression was higher in both groups compared with HD. (D) Absolute number of TIM-3⁺CD4⁺ T cells was higher in the patients in MolR group than that in patients in the TFR group. (E) TIM-3 as a proportion of CD8⁺T cells was higher in the patients in MolR group than in HD. (F) The Absolute counts of CD8⁺T cells was higher in the MolR group. (G) T-regs of patients in the MolR group had higher TIM-3 expression than T-regs of patients in TFR group and HD T-regs. There was no difference between the patients in TFR group and HD. (H) Absolute number of T-regs were higher in patients in the MolR group. There was no difference in TIM-3 expression on NK cells either (I) as a proportion of NK cells or (J) absolute number of TIM-3⁺ NK cells. The Mann-Whitney test was used to compare the 2 groups, whereas multiple comparisons were made using the Kruskal-Wallis test; alpha was set at 0.05 (adjusted for multiple comparisons), ∗∗∗∗P < .0001, ∗∗∗P < .001, ∗∗P < .01. ∗P < .05.