Coexpression of TIM-3 and PD-1 on CD8⁺T cells in patient with CML and in HD. (A) PD-1⁻TIM-3⁺CD8⁺ T cells as a percentage of CD8⁺ T cells were significantly higher in both the patients in the MolR group and those in the TFR group compared with healthy donors (HD). (B) The absolute counts of PD-1⁻TIM-3⁺CD8⁺ T cells was higher in patients in the MolR group compared with those in the TFR group. (C) PD-1⁺TIM-3⁻CD8⁺ T cells as percentage of CD8⁺ T cells or (D) absolute counts of PD-1⁺TIM-3⁻CD8⁺ T cells were not different between the groups. (E) PD-1⁺TIM-3⁺CD8⁺ T cells as a percentage of CD8⁺ T cells was higher in patients in the MolR group compared with HD. (F) Absolute counts of PD-1⁺TIM-3⁺CD8⁺ T cells were higher in patients in the MolR group. (G) Conversely, PD-1⁻TIM-3⁻CD8⁺ T cells were significantly lower in patients in the MolR group than in the HD group. (H) There was no difference in the absolute counts of PD-1⁻TIM-3⁻CD8⁺ T cells. The Mann-Whitney test was used to compare 2 groups, whereas multiple comparisons were made using the Kruskal-Wallis test; alpha was set at 0.05 (adjusted for multiple comparisons).