Multivariable analysis for LFS and OS and transcriptomic implication of high vs low bone marrow plasma S100A8/A9. (A) Multivariable analysis for LFS and OS. Statistically significant if P ≤ .007 (adjusted by Bonferroni correction). ∗As continuous variable analysis. †IPSS-R risk groups: very low, low, intermediate, high, very high. ‡High vs low S100A8/A9. §adjusted with different treatments: supportive care only, HSCT with or without any other treatment, hypomethylating agent with or without other chemotherapies but HSCT, and all other treatments. Note: only variables with P value ≤.05 in univariate analysis were incorporated into the multivariable Cox proportional hazard regression analysis. (B) CIBERSORTx analysis revealing significant differences in the fractions of specific cell types between higher- and lower-S100A8/A9 BM. There were higher proportions of activated CD4 T-cells, CD8 T-cells, NK cells, and naïve B-cells in the lower-S100A8/A9 BM, whereas the fractions of naïve CD4 T-cell, resting mast cells, monocytes, and neutrophils were higher in the higher-S100A8/A9 BM. (C,D) Bar charts showed 10 robustly enriched functional pathways in lower-S100A8/A9 (panel C) and higher-S100A8/A9 (panel D) BMs, respectively. CI, confidence interval; HR, hazard ratios; HSCT, hematopoietic stem cell transplant.