FVII-induced Sox9 expression is dependent on EPCR but independent of TF and PARs. (A) Control and TF CRISPR-edited cells were analyzed for CCL2 and Sox9 expression using qPCR (n = 3). (B) Control and TF CRISPR-edited cells were analyzed for Sox9 expression using western blot. (C) Effect of rFVIIa on Sox9 mRNA levels in TF-deficient MDA^FVII cells. (D) Effect of rFVIIa on Sox9 protein levels in TF-deficient MDA^pcDNA and MDA^FVII cells. (E) Effect of pharmacological PAR inhibitors on Sox9 expression. (F) PAR2 activation in HEK293 cells transfected with vector control or a FVII expression construct, as measured by SEAP release (n = 3). (G) Effect of an EPCR-blocking antibody on Sox9 and Slug expression. (H) Knockdown of the PROCR gene encoding EPCR (n = 3). (I) Effect of PROCR knockdown on Sox9 expression (n = 3). Statistically significant differences were tested using t tests. Graphs show the mean and SD.