American Society of Hematology

Figure 1.

$Conformation plots of SEC-fractionated VWF/fVIII complexes. VWF/fVIII complexes fractionated by Sephacryl S-1000 SEC at pH 6.2 or pH 7.4 were analyzed by SEC MALS to obtain estimates of the weight-average molecular weight and radius of gyration, and by DLS to obtain estimates of the z-average diffusion coefficient. The results are plotted as log10 ⟨Rg⟩z vs log10 Mw (A) or log10 (Dz)20w vs log10 Mw (B). The units of Dz are Ficks (1 F = 10−7 cm2/s). Further shown are the fitted simple linear regression lines and Mark-Houwink-Kuhn-Sakurada exponents with standard errors obtained from the slopes of the regression lines calculated using Prism (version 9.3.0).$

Conformation plots of SEC-fractionated VWF/fVIII complexes. VWF/fVIII complexes fractionated by Sephacryl S-1000 SEC at pH 6.2 or pH 7.4 were analyzed by SEC MALS to obtain estimates of the weight-average molecular weight and radius of gyration, and by DLS to obtain estimates of the z-average diffusion coefficient. The results are plotted as log₁₀ ⟨R_g⟩_z vs log₁₀ M_w (A) or log₁₀ (D_z)_20w vs log₁₀ M_w (B). The units of D_z are Ficks (1 F = 10⁻⁷ cm²/s). Further shown are the fitted simple linear regression lines and Mark-Houwink-Kuhn-Sakurada exponents with standard errors obtained from the slopes of the regression lines calculated using Prism (version 9.3.0).

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