American Society of Hematology

Figure 2.

$Ratios of equivalent radii(aG/aT) of SEC-fractionated VWF/fVIII complexes. (A) The equivalent radii, aG and aT, of VWF/fVIII fractionated at pH 6.2 or pH 7.4 by Sephacryl S-1000 SEC were calculated using the values of ⟨Rg⟩z and (Dz)20w using equations 1 and 2. The dimensionless ratio (aG/aT) is plotted vs estimates of Mw obtained by MALS. The horizontal lines corresponding to aG/aT ratios for spheres and rods were calculated as described.20 The shaded region corresponds to the range of aG/aT ratios expected for a random coil, in which upper and lower limits represent the presence and absence of excluded volume, respectively.20 (B) Cartoon of a pearl string random coil polymer. The more localized distribution of mass for the conformations on the right corresponds to a decreased radius of gyration at pH 6.2.$

Ratios of equivalent radii(a_G/a_T) of SEC-fractionated VWF/fVIII complexes. (A) The equivalent radii, a_G and a_T, of VWF/fVIII fractionated at pH 6.2 or pH 7.4 by Sephacryl S-1000 SEC were calculated using the values of ⟨R_g⟩_z and (D_z)_20w using equations 1 and 2. The dimensionless ratio (a_G/a_T) is plotted vs estimates of M_w obtained by MALS. The horizontal lines corresponding to a_G/a_T ratios for spheres and rods were calculated as described.²⁰ The shaded region corresponds to the range of a_G/a_T ratios expected for a random coil, in which upper and lower limits represent the presence and absence of excluded volume, respectively.²⁰ (B) Cartoon of a pearl string random coil polymer. The more localized distribution of mass for the conformations on the right corresponds to a decreased radius of gyration at pH 6.2.

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