Figure 7.
Ifnar1 deletion causes a modest reduction in T-cell potential in neonatal mice. (A) Clusters 4 and 16 were modestly depleted in Ifnar1–/–- neonates as compared with Ifnar1+/− neonates. Cluster 4 cells expressed genes consistent with HSC identity. Cluster 16 cells expressed some genes associated with myeloid bias (eg, Cd33) and many genes associated with T-lymphoid bias (eg, Notch, Hes1, Runx3, and so on). (B) Reactome pathway analysis of marker genes for cluster 16 showed interferon-related genes as well as Notch/Runx3-associated genes that would align with T-cell bias. (C,D) Results from limiting dilution assays of LK cells (C) or MPP4 (D) plated on OP9-delta stroma to assess T-cell potential at P0. The frequencies of LK and MPP4 cells with T-cell potential in Ifnar1+/- and Ifnar1–/– mice were calculated and compared with the findings from ELDA, n = 48-96 wells per cell dose and genotype across ∼2 to 3 independent experiments.

Ifnar1 deletion causes a modest reduction in T-cell potential in neonatal mice. (A) Clusters 4 and 16 were modestly depleted in Ifnar1–/–- neonates as compared with Ifnar1+/− neonates. Cluster 4 cells expressed genes consistent with HSC identity. Cluster 16 cells expressed some genes associated with myeloid bias (eg, Cd33) and many genes associated with T-lymphoid bias (eg, Notch, Hes1, Runx3, and so on). (B) Reactome pathway analysis of marker genes for cluster 16 showed interferon-related genes as well as Notch/Runx3-associated genes that would align with T-cell bias. (C,D) Results from limiting dilution assays of LK cells (C) or MPP4 (D) plated on OP9-delta stroma to assess T-cell potential at P0. The frequencies of LK and MPP4 cells with T-cell potential in Ifnar1+/- and Ifnar1–/– mice were calculated and compared with the findings from ELDA, n = 48-96 wells per cell dose and genotype across ∼2 to 3 independent experiments.

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