ADAR2 specifically represses the clonogenic growth of CBF AML cells dependent on the RNA editing capability of ADAR2. (A) Bar chart presents reverse transcription-qPCR analysis of ADAR2 expression in Kasumi-1, SKNO-1, ME-1, CTS, and NB4 cells. The relative expression was calculated as described in supplemental Materials and Methods. Data were presented as the mean ± SD of technical replicates from a representative experiment. (B) Western blot analysis of ADAR2 protein expression in Kasumi-1, SKNO-1, ME-1, CTS, and NB4 cells. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. The numbers below the lanes represent the relative protein level, which was determined by the band intensity using Photoshop CC software and normalized to the value of Kasumi-1. Western blot analysis of ADAR2 protein in Kasumi-1 (C), SKNO-1 (E), ME-1 (G), NB4 (I), and CTS (K) cells stably expressing the WT or mutant form of ADAR2 or the EV control via retroviral transduction. GAPDH or actin was used as a loading control. (D,F,H,J,L) Bar chart illustrating the number of colonies formed by the indicated cell groups described in panels C, E, G, I, and K, respectively. Data were presented as the mean ± SD of technical replicates from a representative experiment. (∗∗P < .01; ∗∗∗P < .001 using two-tailed Student t test.)