Figure 1.
The landscape of IL-7R expression in normal and malignant thymocytes. (A) Surface IL-7Rα chain (CD127 or sIL-7R) expression was analyzed by flow-cytometry (FC) on gated thymocytic subpopulations from human thymi (n = 3), as described in “Methods.” (Left) A representative example is shown for a single thymus. (Right) For each thymus, the median fluorescence intensity (MFI) of each thymocyte subpopulation was normalized to DP CD3+ thymocytes median fluorescence intensity. The ratio of fluorescence intensity is shown for each thymocytic subpopulation. The mean and standard error of the mean are shown. (B) (Left) IL-7R expression was analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) on thymocytic subpopulations sorted from 2 independent human thymi. IL-7R transcript level is expressed as the percentage of ABL1 endogenous control. Means and SEM are shown. Kruskal-Wallis test with post hoc Dunn multiple comparisons. (Right) IL-7R expression was assessed using bulk RNA-seq on sorted thymic subpopulations from 1 human thymus. Normalized log2 expression values are shown. (C) IL-7R expression was analyzed in a cohort of adult T-ALL cases using qRT-PCR (n = 146) and bulk RNA-seq (n = 156) as in panel B. T-ALL were classified based on the immunophenotype. Means and SEM are shown. Kruskal-Wallis test with post hoc Dunn multiple comparisons. ∗∗P < .01. (D) CD127 (sIL-7R) expression was assessed via FC in 200 adults and 100 pediatric unselected T-ALL cases. The bar graph depicts the proportion of sIL-7R+ (orange) and sIL-7R− (gray) cases in each group. Fisher's exact test. (E) (Left) sIL-7R expression level (percentage of CD127+ blasts) for each T-ALL from the adult cohort classified based on their immunophenotype (n = 198). Means and SEM are shown. Kruskal-Wallis test with post hoc Dunn multiple comparisons. (Right) Relative proportions of sIL-7R+ and sIL7R–/low T-ALL from the adult cohort (n = 198) for each immunophenotypic subgroup. The threshold of positivity was set at 20%. χ2 test. ∗∗∗∗P < .0001. DN, double negative; DP, double positive; ISP, immature single positive; SP, single positive.

The landscape of IL-7R expression in normal and malignant thymocytes. (A) Surface IL-7Rα chain (CD127 or sIL-7R) expression was analyzed by flow-cytometry (FC) on gated thymocytic subpopulations from human thymi (n = 3), as described in “Methods.” (Left) A representative example is shown for a single thymus. (Right) For each thymus, the median fluorescence intensity (MFI) of each thymocyte subpopulation was normalized to DP CD3+ thymocytes median fluorescence intensity. The ratio of fluorescence intensity is shown for each thymocytic subpopulation. The mean and standard error of the mean are shown. (B) (Left) IL-7R expression was analyzed using quantitative reverse transcription polymerase chain reaction (qRT-PCR) on thymocytic subpopulations sorted from 2 independent human thymi. IL-7R transcript level is expressed as the percentage of ABL1 endogenous control. Means and SEM are shown. Kruskal-Wallis test with post hoc Dunn multiple comparisons. (Right) IL-7R expression was assessed using bulk RNA-seq on sorted thymic subpopulations from 1 human thymus. Normalized log2 expression values are shown. (C) IL-7R expression was analyzed in a cohort of adult T-ALL cases using qRT-PCR (n = 146) and bulk RNA-seq (n = 156) as in panel B. T-ALL were classified based on the immunophenotype. Means and SEM are shown. Kruskal-Wallis test with post hoc Dunn multiple comparisons. ∗∗P < .01. (D) CD127 (sIL-7R) expression was assessed via FC in 200 adults and 100 pediatric unselected T-ALL cases. The bar graph depicts the proportion of sIL-7R+ (orange) and sIL-7R− (gray) cases in each group. Fisher's exact test. (E) (Left) sIL-7R expression level (percentage of CD127+ blasts) for each T-ALL from the adult cohort classified based on their immunophenotype (n = 198). Means and SEM are shown. Kruskal-Wallis test with post hoc Dunn multiple comparisons. (Right) Relative proportions of sIL-7R+ and sIL7R–/low T-ALL from the adult cohort (n = 198) for each immunophenotypic subgroup. The threshold of positivity was set at 20%. χ2 test. ∗∗∗∗P < .0001. DN, double negative; DP, double positive; ISP, immature single positive; SP, single positive.

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