CEBPD overexpression and a DS genetic background contribute to differentiation to the pro-B stage demonstrated using scRNA-Seq of CEBPD and control transduced WT and Dp16 murine cells. (A) UMAP projection and clustering of cells from all 4 conditions. (B) Expression of hematopoietic differentiation marker genes assigned the 13 clusters to 4 cell types. (C) UMAP projection and cell differentiation stage of the 4 conditions. (D) The frequencies of each cell type in the clusters across the 4 conditions. (E) Cell cycle analysis of the cells and the percentages of pro-B cells in each cell cycle phase across the 4 experimental conditions.