Figure 2.
MC5R deficiency aggravates myelosuppression after radiation injury. (A) Enzyme-linked immunosorbent assay of α-MSH level in the BM supernatant of WT mice at the indicated time after 5.0 Gy total body irradiation (TBI) (n = 5). Unirradiated WT mice were used as controls (n = 5). (B) qRT-PCR analysis of MC5R expression in LSKs sorted from the BM of WT mice at the indicated time after 5.0 Gy TBI (n = 3). The relative expression of MC5R was compared with that at 0 hour. (C-E) The counts of (C) WBC, (D) RBC, and (E) PLT in the PB of WT and MC5R–/– mice at the indicated time after 5.0 Gy TBI (n = 9). (F) Total number of BM cells in WT and MC5R–/– mice at the indicated time after 5.0 Gy TBI (n = 6). (G) Representative flow cytometric plots showing the percentages of MPs, LSKs, LT-HSCs, ST-HSCs, and MPPs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI. (H) The numbers of MPs and LSKs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI (n = 6). (I) The number of LT-HSCs, ST-HSCs, and MPPs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI (n = 6). (J) The number of MEPs, CMPs, GMPs, and CLPs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI (n = 6). (K) Flow cytometric analysis of the percentages of T cells, B cells, and myeloid cells in the BM of WT and MC5R-/- mice 15 days after 5.0 Gy TBI (n = 6). (A,B) one-way ANOVA with Tukey multiple comparisons test; (C-F,H-K) unpaired t test (two-tailed). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Con, control; IR, irradiation.

MC5R deficiency aggravates myelosuppression after radiation injury. (A) Enzyme-linked immunosorbent assay of α-MSH level in the BM supernatant of WT mice at the indicated time after 5.0 Gy total body irradiation (TBI) (n = 5). Unirradiated WT mice were used as controls (n = 5). (B) qRT-PCR analysis of MC5R expression in LSKs sorted from the BM of WT mice at the indicated time after 5.0 Gy TBI (n = 3). The relative expression of MC5R was compared with that at 0 hour. (C-E) The counts of (C) WBC, (D) RBC, and (E) PLT in the PB of WT and MC5R–/– mice at the indicated time after 5.0 Gy TBI (n = 9). (F) Total number of BM cells in WT and MC5R–/– mice at the indicated time after 5.0 Gy TBI (n = 6). (G) Representative flow cytometric plots showing the percentages of MPs, LSKs, LT-HSCs, ST-HSCs, and MPPs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI. (H) The numbers of MPs and LSKs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI (n = 6). (I) The number of LT-HSCs, ST-HSCs, and MPPs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI (n = 6). (J) The number of MEPs, CMPs, GMPs, and CLPs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI (n = 6). (K) Flow cytometric analysis of the percentages of T cells, B cells, and myeloid cells in the BM of WT and MC5R-/- mice 15 days after 5.0 Gy TBI (n = 6). (A,B) one-way ANOVA with Tukey multiple comparisons test; (C-F,H-K) unpaired t test (two-tailed). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001. Con, control; IR, irradiation.

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