Figure 5.
MC5R regulates HSPC proliferation via the PI3K/AKT and MAPK pathways. (A) GSEA of RNA-seq data showing cytokine signaling-associated enrichment in LSKs from irradiated WT and MC5R–/– mice. (B-D) Flow cytometric analysis of the expression of (B) p-PI3K, (C) p-AKT, and (D) p-ERK1/2 in LSKs and LT-HSCs from the BM of WT and MC5R-/- mice 15 days after 5.0 Gy TBI (n = 6). Representative flow cytometric plots (left). (E-I) Flow cytometric analysis of the expression of (E) p-STAT1, (F) p-STAT3, (G) p-STAT5, (H) p-Smad2/3, and (I) p-p65 in LSKs and LT-HSCs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI (n = 6). (J,K) Representative immunofluorescence plots showing (J) p-AKT and (K) p-ERK1/2 expression in LSKs sorted from the BM of WT and MC5R–/– mice at 15 days after 5.0 Gy TBI. (B-I) unpaired t test (two-tailed). ∗∗∗P < .001.

MC5R regulates HSPC proliferation via the PI3K/AKT and MAPK pathways. (A) GSEA of RNA-seq data showing cytokine signaling-associated enrichment in LSKs from irradiated WT and MC5R–/– mice. (B-D) Flow cytometric analysis of the expression of (B) p-PI3K, (C) p-AKT, and (D) p-ERK1/2 in LSKs and LT-HSCs from the BM of WT and MC5R-/- mice 15 days after 5.0 Gy TBI (n = 6). Representative flow cytometric plots (left). (E-I) Flow cytometric analysis of the expression of (E) p-STAT1, (F) p-STAT3, (G) p-STAT5, (H) p-Smad2/3, and (I) p-p65 in LSKs and LT-HSCs in the BM of WT and MC5R–/– mice 15 days after 5.0 Gy TBI (n = 6). (J,K) Representative immunofluorescence plots showing (J) p-AKT and (K) p-ERK1/2 expression in LSKs sorted from the BM of WT and MC5R–/– mice at 15 days after 5.0 Gy TBI. (B-I) unpaired t test (two-tailed). ∗∗∗P < .001.

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