USP1 is overexpressed in T-ALL. (A) Scatter dot plots showing the mRNA levels of USP1 in normal BM from healthy donors and primary T-ALL (73 normal BM and 169 T-ALL samples). Data were obtained from the GSE13159. Two-sided Mann-Whitney tests. (B) Analysis of USP1 expression in 231 primary pediatric ALL samples that are categorized into 7 different subtypes in the Ma-Spore ALL study cohort (Wilcoxon rank-sum test, T-ALL vs B-ALL, P<0.0001). (C) Cumulative relapse incidence in 36 pediatric patients with T-ALL; P = .034. (D-E) Immunoblots of USP1 in 20 primary pediatric T-ALL samples are indicated. Representative immunoblotting image of USP1 is shown in panel D. Four samples from pediatric patients with immune thrombocytopenic purpura (ITP) were used as negative controls. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serves as a loading control. The relative protein expression levels of USP1 are shown in panel E. Two-sided Mann-Whitney tests; ∗∗P < .01, ∗∗∗P < .001. FPKM, fragments per kilo base per million mapped reads.