Figure 5.
USP1 promotes cell proliferation and glycolysis via PLK1-LDHA axis. (A) Jurkat cells were infected with vector or USP1-flag lentivirus. Then the USP1 overexpression group was treated with BI2536 (30 nM) for 48 hours. CCK-8 was used to assess the cell proliferation. (B) The lactate production was tested. Data are shown as mean ± SD, n = 3. (C) The expression of USP1 and LDHA was measured. (D) Jurkat cells were infected with USP1 sh-control, USP1 sh-1, or cotransduced with LDHA-flag or USP1-cherry lentivirus. Then CCK-8 was used to assess the cell proliferation. (E) Jurkat cells were infected with USP1 sh-control, USP1 sh-1, or cotransduced with LDHA-flag or USP1-cherry lentivirus. The lactate production was tested. Data are shown as mean ± SD, n = 3. ∗∗P < .01, ∗∗∗P < .001. (F) The expression of USP1 and LDHA was measured. OD, optical density.

USP1 promotes cell proliferation and glycolysis via PLK1-LDHA axis. (A) Jurkat cells were infected with vector or USP1-flag lentivirus. Then the USP1 overexpression group was treated with BI2536 (30 nM) for 48 hours. CCK-8 was used to assess the cell proliferation. (B) The lactate production was tested. Data are shown as mean ± SD, n = 3. (C) The expression of USP1 and LDHA was measured. (D) Jurkat cells were infected with USP1 sh-control, USP1 sh-1, or cotransduced with LDHA-flag or USP1-cherry lentivirus. Then CCK-8 was used to assess the cell proliferation. (E) Jurkat cells were infected with USP1 sh-control, USP1 sh-1, or cotransduced with LDHA-flag or USP1-cherry lentivirus. The lactate production was tested. Data are shown as mean ± SD, n = 3. ∗∗P < .01, ∗∗∗P < .001. (F) The expression of USP1 and LDHA was measured. OD, optical density.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal