Ehbp1l1^−/− mice exhibit lethal anemia. (A) The numbers of progenies with the indicated genotypes from Ehbp1l1^+/− intercrosses are shown. (B) Survival curves of the progenies from Ehbp1l1^+/− intercrosses are shown (Ehbp1l1^+/+: n = 18, Ehbp1l1^+/−: n = 47, Ehbp1l1^−/−: n = 19). (C,E) Representative pictures of Ehbp1l1^+/− and Ehbp1l1^−/− mice at P0 (the day of birth) (C) and E18.5 (E) are shown. (D,F) Total cell numbers in peripheral blood at P0 (D, Ehbp1l1^+/+ shown as a solid green circle: n = 1, Ehbp1l1^+/− shown as open green circles: n = 5, Ehbp1l1^−/−: n = 6) and E18.5 (F, Ehbp1l1^+/+: n = 5, Ehbp1l1^+/−: n = 9, Ehbp1l1^−/−: n = 4) are shown. The data are presented as the means ± standard error of the mean (SEMs). (G) Whole-cell extracts (WCEs) from fetal livers (FL) of E15.5 embryos with the indicated genotypes were analyzed by western blotting using the indicated antibodies. (H) WCEs from Ehbp1l1^−/− mouse embryonic fibroblasts transfected with pcDNA3 encoding EHBP1L1 isoform B, C, E, or X5 and WCEs from E14.5 mouse FL were subjected to western blotting. ∗∗∗P < .001, ∗∗∗∗P < .0001 (unpaired t test with Welch’s correction in panel D; 1-way analysis of variance [ANOVA] in panel F).