EHBP1L1 promotes erythroblast enucleation. (A) The peripheral blood collected from control and Ehbp1l1^−/− P0 mice was subjected to May-Grunwald-Giemsa staining. Representative pictures are shown. The areas enclosed in the black squares were enlarged and are shown in the right panels. Scale bars: 10 μm. (B-C) The peripheral blood collected from mice with the indicated genotypes was analyzed by flow cytometry (Ehbp1l1^+/+: n = 5, Ehbp1l1^+/−: n = 9, Ehbp1l1^−/−: n = 4). Representative flow cytometry plots are shown. (D-E) Fetal liver cells (FLCs) from control and Ehbp1l1^−/− embryos were analyzed by flow cytometry. N =14 (E13.5), 16 (E14.5), 7 (E15.5), 3 (E16.5), 5 (E17.5), and 9 (E18.5) for control embryos. N =5 (E13.5), 4 (E14.5), 5 (E15.5), 2 (E16.5), 4 (E17.5), and 3 (E18.5) for Ehbp1l1^−/− embryos. The upper and lower flow cytometry plots in E show representative data for Lin⁻Ter119^highHoechst 33342⁺AnnexinV⁻ erythroblasts and Lin⁻Ter119^high cells, respectively. (F-G) FLCs from E13.5 ICR embryos were analyzed by flow cytometry (n = 4). Representative flow cytometry plots of erythroid cells at distinct stages of erythropoiesis are shown. The mean fluorescence intensity (MFI) of EHBP1L1 staining was normalized based on that of control rabbit IgG staining. The result is representative of 2 independent experiments. The data are presented as means ± SEMs for panels C-D,G. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001, ∗∗∗∗P < .0001 (1-way ANOVA in panels C,G; unpaired t test with Welch’s correction in panel D). BFU-E, burst-forming units-erythroid; CFU-E, colony-forming units-erythroid; ProE, proerythroblasts.