Mice transplanted with Ehbp1l1^−/− hematopoietic cells exhibited impaired erythroblast enucleation and hemolytic anemia. FLCs from Ehbp1l1^+/+ and Ehbp1l1^−/− E14.5 embryos were transplanted into irradiated wild-type mice. (A) Total cell numbers in the peripheral blood at indicated days after transplantation (control: n = 5, Ehbp1l1^−/−: n = 4). (B-E) Peripheral blood (B-C) and bone marrow cells (D-E) collected at indicated (B-C) and 35 days (D-E) after transplantation, respectively, were analyzed by flow cytometry (control: n = 5, Ehbp1l1^−/−: n = 4). Representative flow cytometry plots are shown (Lin⁻ cells in panel D). (F-H,K) The livers collected 35 days after transplantation were subjected to H&E staining (F), immunohistochemistry for Ter119 (G), and Berlin blue staining (K). The peripheral blood collected 22 days after transplantation was subjected to May-Grunwald-Giemsa staining (H). Representative pictures are shown. The areas enclosed in the black squares were enlarged (F,K). Arrows indicate stomatocytes (H). (I) A representative picture of the spleens collected 35 days after transplantation is shown. (J) The bilirubin concentration in the serum at indicated days after transplantation was determined by using the UnaG protein (control: n = 4 and Ehbp1l1^−/−: n = 4). Scale bars: 200 and 50 μm in enlarged pictures for panels F,K, 10 μm for panels G-H, 1 cm for panel I. The data are presented as means ± SEMs for panels A,C,E,J. ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001 (2-way ANOVA in panels A,C,J; unpaired t test with Welch’s correction in panel E).