Transferrin and transferrin receptor interaction as a potential therapeutic target in ANKL. (A) Schema for calculating the interaction score. The top 100 expressed genes in the human liver were extracted from the GTEx database. The interactome is based on the HuRI. (B) Liver-ANKL interaction associated with Myc regulation. (C) TF gene expression among major human tissues according to the GTEx data. (D) Transferrin receptor 1 (TfR1) expression in normal NK cells derived from healthy donor, ANKL1, ANKL3, and ANKL5 cells analyzed by flow cytometry. (E) Expression profile of iron-related genes in ANKL1-PDX compared with that in normal NK cells. Genes are classified into the following groups: group 1, iron uptake; group 2, iron storage; group 3, iron efflux; group 4, iron homeostasis; group 5, heme biosynthesis; group 6, cytosolic iron-sulfur cluster protein assemble; and group 7, mitochondrial iron-sulfur cluster protein biogenesis or export. (F) Expression profile of iron-related genes in the hepatic niche of PDX mice compared with that in the normal liver. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.