Nrf2 expression affects histone methylation in SCD. (A) Histone methylation and NRF2 levels in EPs from HD and patients with SCD. EPs from HDs and patients with SCD were cultured in a modified Fibach 2-phase culture system, and on day 12 of culture, they were determined using the indicated histone and NRF2 antibodies. (B) Histone methylation in the histone extracts of spleen Ter119⁺ cells from SS/Nrf2^+/+ and SS/Nrf2^–/– mice. (C) Histone methylation in 12-day EPs from patients with SCD cultured under normoxia (21% O₂), treated with H₂O₂ (200 μM) and hypoxia (1% O₂), H₂O₂. (D) Histone methylations in non-SCD C57BL/6 mice were determined using the indicated antibodies in the spleen Ter119⁺ cell histone extracts of adult Nrf2^+/+ and Nrf2^–/– mice. Total histone H3 was used as a loading control. Data represent mean ± SD; n = 3 to 6. ∗P < .05. HD, healthy donor.