Figure 2. Clonal evolution of somatic... | American Society of Hematology

Figure 2.

$Clonal evolution of somatic mutations in CLL patients with concomitant loss(8p) and gain (1q21.2). Subclonal structure and clonal evolution of somatic mutations derived using PhylogicNDT (left, middle) and Concerti (right) for CLL1 (A) and CLL2 (B). (Left) Each line represents the cancer cell fraction (CCF) of mutation clusters with shading representing a 95% confidence interval. Each dot represents a different time point. Phylogenetic trees (middle) represent the best estimated clonal and subclonal architecture corresponding to the same clones (represented in the same colors) as in the left panels. (Right) Time-scaled Concerti plots. Clones are sized proportionally to their prevalence and represented by the same color as in the PhylogicNDT plots. Copy number data are shown under the PhylogicNDT plots, as derived from ABSOLUTE in acquired patients with loss(8p) and gain(1q21.2) (CLL1 and CLL2). See Figure 3 and supplemental Figure 2 for the remaining patients. (C) Multiplexed fluorescence in situ hybridization performed on pre- and postprogression venetoclax samples for CLL1 and CLL2. The probe combination was tel8p (green) and tel8q (red) for the first column, MCL1 (red) and CCP1 (green) for the second column, and a combination of MCL1 (red) denoted by red arrows, CCP-1 (green) denoted by white arrows and tel8p (green) denoted by yellow arrows for the third column.$

Clonal evolution of somatic mutations in CLL patients with concomitant loss(8p) and gain (1q21.2). Subclonal structure and clonal evolution of somatic mutations derived using PhylogicNDT (left, middle) and Concerti (right) for CLL1 (A) and CLL2 (B). (Left) Each line represents the cancer cell fraction (CCF) of mutation clusters with shading representing a 95% confidence interval. Each dot represents a different time point. Phylogenetic trees (middle) represent the best estimated clonal and subclonal architecture corresponding to the same clones (represented in the same colors) as in the left panels. (Right) Time-scaled Concerti plots. Clones are sized proportionally to their prevalence and represented by the same color as in the PhylogicNDT plots. Copy number data are shown under the PhylogicNDT plots, as derived from ABSOLUTE in acquired patients with loss(8p) and gain(1q21.2) (CLL1 and CLL2). See Figure 3 and supplemental Figure 2 for the remaining patients. (C) Multiplexed fluorescence in situ hybridization performed on pre- and postprogression venetoclax samples for CLL1 and CLL2. The probe combination was tel8p (green) and tel8q (red) for the first column, MCL1 (red) and CCP1 (green) for the second column, and a combination of MCL1 (red) denoted by red arrows, CCP-1 (green) denoted by white arrows and tel8p (green) denoted by yellow arrows for the third column.

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