Hepcidin expression levels are unaffected by HIF-PHIs in the ISAM and AnRED mutant mouse lines.Hamp (hepcidin) and Fam132b (erythroferrone) mRNA expression in the livers, bone marrow, and spleens was analyzed in the control, ISAM, or AnRED mice at 6 hours after injection of GSK360A (50 mg/kg) or roxadustat (Rox, 200 mg/kg). In each graph, the average expression levels in the vehicle-treated control mice were normalized to 1. ∗P< .05 or ∗∗P < .01 calculated by Student t test (Control and AnRED) or Tukey-Kramer test (ISAM).