Effects of infused DGKKO in HIT-induced thrombocytopenia in a mice model. Studies were performed in HIT mice after IP injection of HIT-like monoclonal antibody or HIT IgGs to induce thrombocytopenia and was followed by IP (A) or IV (B) injection of DGKKO at the indicated times. The number of independent experiments for each condition is indicated in the figures. Mean ± 1 SEM of relative platelet counts compared with the baseline are shown; N values are included in each study. Gray, dashed line refers to baseline platelet count before the study. (A) Relative platelet counts after an IP infusion of KKO followed by an IP injection of DGKKO. (B) Same as in panel A, but DGKKO was administered IV. (C) Same as in panel B, but 5B9 was administered instead of KKO. (D,E) Studies were performed as in panel B using IgG isolated from the plasma of 2 patients with HIT. (F) Studies were performed similarly but after fondaparinux (20 μg per mouse) was given IV either 1 or 6 hours after KKO induction of thrombocytopenia.