Effect of DGKKO and fondaparinux on thrombosis induced by KKO in a murine model of HIT. Cremaster arteriole injury followed by infused KKO. HIT prothrombotic studies done as outlined in supplemental Figure 2C. (A) (Top) Representative image of 5 minutes after injury vs 15 minutes after KKO infusion vs 30 minutes after DGKKO infusion on platelet and fibrin accumulation. Scale bar is included, as is the arrow indicating the direction of flow. (Middle) Quantitative analysis of platelet accumulation at the site of injury before and after KKO and then after DGKKO. Mean ± 1 SEM are shown. P values were determined using 2-way Student t test, comparing platelet accumulation after KKO vs after DGKKO. (Bottom) Same as the middle graph but for fibrin accumulation. (B) Same as panel A but DGTRA-infused after KKO rather than after DGKKO. (C) Cremaster arteriole injury followed by KKO or KKO + fondaparinux. Data are expressed as the fold change of platelet accumulation and fibrin deposition after KKO (1 mg/kg) or KKO + fondaparinux injection (1 μg/kg) compared with that from before the therapy. Values greater than 1 are consistent with a prothrombotic effect. n = 3 studies per arm; P values were determined using Student t test.