GOLM1 is overexpressed and correlates with ALK in human ALK⁺ ALCL. (A) Correlation of the expression levels of GOLM1 and ALK. Colors are z score normalized to depict relative values within rows. (B) The GOLM1 transcript (calculated as log2 median-centered intensity) as studied in Human Genome U133 Plus 2.0 Array platform in samples from patients with ALK⁺ ALCL from the Oncomine database (Eckerle lymphoma) and compared with normal T lymphocyte/NK cells and ALK^- ALCL. (C) Correlation between mRNA transcript levels of ALK and GOLM1 in human samples from patients with ALCL from Oncomine database. (D) A representative image of immunohistochemistry analysis to show the expression of GOLM1 in ALK⁺ ALCL, but not in samples from patients with ALK^- ALCL. Original magnification 200×. (E-F) GOLM1 mRNA and protein expression in the ALK⁺ ALCL cell lines DEL, SUP-M2, Karpas 299, SUDHL-1, and other cell lines was evaluated by qPCR and western blotting, respectively. Data, presented as the mean ± SD, are representative of 3 independent experiments. NK, natural killer; SD, standard deviation.