GOLM1 is upregulated by NA. (A) The ALK inhibitor reduces the transcription level of GOLM1 in ALK⁺ ALCL cells. The total RNA from ALK⁺ ALCL (SUP-M2 and Karpas 299) and Jurkat T-cell lines with or without treatment of the ALK inhibitors CEP28122 (100 nM)⁴¹ or crizotinib (2 μM)⁴² for 24 hours were subjected RT-qPCR analysis. (B) The ALK inhibitor reduces the protein level of GOLM1 in ALK⁺ ALCL cells. The whole cell lysate from ALK⁺ ALCL (SUP-M2 and Karpas 299) and Jurkat cell lines with or without treatment of ALK inhibitor CEP28122 (100 nM) for 24 hours were subjected to western blotting. (C-D) Purified CD4⁺ T cells were stimulated with anti-CD3/CD28 beads and either transduced with wild-type NA or KD or left uninfected, then cultured in CO₂ incubator. Cells were count and expanded according to previous description.¹² GOLM1 mRNA and protein were gradually increased during human T-cell transformation process upon lentivirus-encoded NA (Lenti-NA) infection. Samples were collected at 0, 7, 14, 28, 35, and 49 days after infection. (E) The mRNA and protein levels of GOLM1 in MyLa2059 and MyLa3675 cells transduced with NA or its KD mutant were individually detected by qPCR and western blot. (F-K) The pan-AKT inhibitor Ipatasertib and mTOR inhibitor rapamycin reduced the transcription and protein levels of GOLM1 in ALK⁺ ALCL cells. The total RNA and cell lysate from ALK⁺ ALCL cell lines with or without treatment of Ipatasertib or rapamycin at indicated concentration for 24 hours were subjected qPCR and western blot analysis. (∗P < .05, ∗∗∗P < .001, 2-tailed Student t test).