Inhibition of GOLM1 expression in ALK⁺ ALCL cells reduces cell viability. (A) Establishment of GOLM1 stable knockdown in ALK⁺ALCL (SUDHL-1, SUP-M2, and Karpas 299) and Jurkat cells. Cells were infected by lentivirus-encoded GOLM1 or scramble shRNA, and stable clones were selected. The GOLM1 mRNA and (B) protein were detected by qPCR or western blotting (∗P < .05, ∗∗∗P < .001, 2-tailed Student t test). (C) The growth curves of GOLM1 knockdown ALK⁺ ALCL cells and (D) NA or KD expressing ALK^- cells. The shGOLM1 or shscramble ALK⁺ ALCL cells were seeded as 1 × 10⁵ per well (200 μL) for expansion. The cells were counted and seeded as 1 × 10⁵ per well (200 μL) every other day during 8 or 10 days period. (E) The viability of GOLM1 knockdown ALK⁺ ALCL cells and (F) NA or KD expressing ALK^- ALCL cells. The cells were seeded as 1 × 10⁵ per well (200 μL). Cell viability was determined by trypan blue staining.