GOLM1 expression increase the expression of BCL-X_L. (A) RNA-seq was performed for shGOLM1 or shscramble SUP-M2 cells and the differentially expressed genes were analyzed and presented as a volcano plot. (B) Functional pathway analysis performed by Database for Annotation, Visualization, and Integrated Discovery and KEGG database, and the top 10 most significant pathway are presented as GO terms. (C) The heat map depicts most changed genes in shGOLM1 RNA-seq data compared with scramble samples. (D) The heat map shows no genes significantly change in apoptosis pathway in in shGOLM1 RNA-seq data compared with scramble samples, which was analyzed and plotted by R project software. (E) SUP-M2 cells with GOLM1 overexpression or control were treated with DMSO or STS, and then the protein levels of PARP-1, BCL-X_L, BCL-2, MCL-1, GOLM1, and cleaved caspase 3 were detected by western blotting. GO, Gene Ontology; MCL, myeloid cell leukemia.