Figure 2.
miR-148a-3p targets DDX6 in leukemia cells. (A) The WT 3′ UTR (ie, contains the binding site of miR-148a-3p) or mutated 3′ UTR-mut (ie, includes the mutated binding site of miR-148a-3p) of DDX6 or RAB12 was inserted downstream of the FL CDS in the reporter plasmid, pGL-FL. (B) Dual-luciferase reporter assays were conducted for DDX6 and RAB12. The FL signal was normalized against the Renilla luciferase signal. (C) miR-148a-3p overexpression in MOLM3 cells (miR148a-3p OV MOLM13). The expression of miR-148a-3p was calculated by qPCR and normalized against U6. (D) qPCR showing reduction of DDX6 mRNA in miR148a-3p OV MOLM13. DDX6 mRNA abundance was calculated by qPCR and normalized against glyceraldehyde-3-phosphate dehydrogenase. (E) Western blot showing a decrease in DDX6 protein in miR148a-3p OV MOLM13. CMV, cytomegalovirus; EV, empty vector.

miR-148a-3p targets DDX6 in leukemia cells. (A) The WT 3′ UTR (ie, contains the binding site of miR-148a-3p) or mutated 3′ UTR-mut (ie, includes the mutated binding site of miR-148a-3p) of DDX6 or RAB12 was inserted downstream of the FL CDS in the reporter plasmid, pGL-FL. (B) Dual-luciferase reporter assays were conducted for DDX6 and RAB12. The FL signal was normalized against the Renilla luciferase signal. (C) miR-148a-3p overexpression in MOLM3 cells (miR148a-3p OV MOLM13). The expression of miR-148a-3p was calculated by qPCR and normalized against U6. (D) qPCR showing reduction of DDX6 mRNA in miR148a-3p OV MOLM13. DDX6 mRNA abundance was calculated by qPCR and normalized against glyceraldehyde-3-phosphate dehydrogenase. (E) Western blot showing a decrease in DDX6 protein in miR148a-3p OV MOLM13. CMV, cytomegalovirus; EV, empty vector.

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