Downregulation of CSF3R expression. (A) CSF3R GE changes over time (log₂-fold change) in cHL-derived cell lines compared with 2 different controls: GAPDH and CD30 (TNFRSF8). (B) CSF3R protein expression measured by IHC (cytospin preparations) to compare untreated (left) with ruxolitinib-treated (right) cell lines. The histogram illustrates the pattern of IHC quantification (two-way ANOVA: ∗∗∗P < .001). ANOVA, analysis of variance.