IL-27 enhances CD38 upregulation during in vitro PC differentiation. (A) PCs were generated from 4 healthy donors either with or without the addition of rIL-27 from day 0 to day 6 of the protocol (see text). The impact of IL-27 on PC differentiation was assessed by comparing the proportion of long-lived PCs on day 20, as determined by CD38 and CD138-positivity via flow cytometry. Representative dot plots and individual values are shown. (B) The expression of CD38 was compared throughout the protocol between B cells grown with or without rIL-27. Representative histograms and values from individual donors are shown. ∗∗∗P < .001; ∗∗∗∗P < .0001 by two-way ANOVA and Sidak multiple comparison test.