CD34⁺cells exhibit natural production of FVIII upon cytokine-induced differentiation in culture. (A-B) FVIII percentage in fresh human CB-derived CD34⁺ HSCs measured as MFI by FC. (A) FC gating strategy and examples of FVIII percentage in different HSC progenitors. (B) FVIII percentage in total CD34⁺ cells and different progenitors (n = 4, mean). (C) FVIII percentage in fresh vs cultured CD34⁺ cells in serum-free medium (n ≥ 4, mean). (D) Representative FC plot of 7 to 10 days cultured CD34⁺ cells. CLP, common lymphoid progenitor; CMP, common myeloid progenitors; FSC, forward scatter; GMP, granulocyte-macrophage progenitor; IgG, immunoglobulin G; MFI, mean fluorescence intensity; MPP, multipotent progenitor.