PBMC myeloid compartment is the richest in FVIII⁺cells in the hematopoietic system. (A) This logical gating strategy was consistently used to analyze FVIII expression in all samples, with gates on PBMCs, singlets, viable cells, and FVIII vs matched isotype control; here, an example of FVIII percentage in total PBMCs. (B) Gating strategy used for selecting PBMC lymphoid and myeloid subsets. (C) Collective analysis of FVIII expression (%) in different PBMC subsets (n ≥ 3, mean). Mac-Mo, macrophage/monocyte; SSC, side scatter.