HA ZF modeling with f8-MO antisense RNA. (A) Representative fluorescence images: (I) merged and zoomed-in image of Tg:gata1:dsRed ZF larvae, injected with 1.0 mM f8-MO and tagged with fluorescein at 1-cell stage and visualized at 72 hours after fertilization. The hematopoietic niche is contoured by a white box (×25 magnification); (II) Green fluorescence zoomed in image of f8-MO localized in the hematopoietic niche as shown by the arrows (original magnification ×112); and (III) Merged image (original magnification ×112) showing transgenic red blood cells (red) and f8-MO (green, merged = orange). (B) Viability percentage of ZF larvae injected with f8-MO. The number of measured ZF is noted on the columns. Significance was calculated on a χ2 test, with a subsequent pairwise post hoc z test (C) Danioscope calculation of the blood flow activity in selected areas of the DA and PCV. The average DA plus PCV blood flow activity (%) of all MO groups was compared with the control group (n ≥ 6, mean). (D) Laser-induced injury and clot formation time of ZF injected with f8-MO (n ≥ 6, mean).
