Exosc3 ablation reduces immunophenotypic BFU-E and KIT cell-surface surface expression. (A) Experimental strategy. (B) Representative flow plots depicting erythroid maturation based on KIT, CD71, and CD24 expression to discriminate BFU-E (Lin⁻KIT⁺CD24/71^10%low) and CFU-E (Lin⁻KIT⁺CD24/71^20%high) populations in Exosc3^C/C Cre⁻ and Exosc3^C/C Cre⁺ E14.5 embryos. (C) Quantification of Lin⁻KIT⁺CD24/71^10%low BFU-E and Lin⁻KIT⁺CD24/71^20%high CFU-E (3 independent experiments: Exosc3^C/C Cre⁻ = 8 and Exosc3^C/C Cre⁺ = 5). (D) GFP percentage in BFU-E gate for Exosc3^C/C Cre⁻, Exosc3^C/WT Cre⁺, and Exosc3^C/C Cre⁺ conditions. Significance by Tukey multiple comparisons test. (E) KIT⁺ cells within Lin⁻ progenitor population. (F) KIT cell-surface expression within Lin⁻ progenitors. (G) KIT cell-surface expression in Lin⁻KIT⁺CD24/71^10%low BFU-E population. Quantitative data are presented as box and whisker plots, with bounds from the 25th to 75th percentiles, the median line, and whiskers ranging from minimum to maximum values. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001, by unpaired t test.