WES of the PC compartment from paired samples. (A) Number of total mutations as well as transitions and transversions from bone marrow and lesion (OL) for each individual patient. No significant differences between lesion and marrow were found for number of mutations and transitions/transversions in both NDMM and RRMM. (B) Significantly more mutations were found in patients with RRMM compared with NDMM in both locations. (C) Scatterplots of variant allele frequencies in the bone marrow (x-axis) and lesion (y-axis) in NDMM (blue) and RRMM (red). Jaccard indices were calculated to quantify overlap between paired samples. The largest numbers of unshared mutations were found in patients with EMD (indicated by black stars). BM, bone marrow.