SCE5-scuPA protects the BBB, reduces cerebral blood content, and attenuates immunosuppression and thrombocytopenia after photothrombotic stroke. (A and B) Albumin extravasation (A) and cerebral hemoglobin (Hb) levels (B) in perfused brains 3 days after photothrombotic stroke in mice treated just before stroke with saline (vehicle), SCE5-scuPA (150 U/g), or TNK-tPA (2.5 mg/kg). The contralateral (Contra) values were subtracted from the ipsilateral (Ipsi) values to correct for perfusion efficiency. (C-E) Under the same protocol settings: WBC counts at 3 and 7 days poststroke (C), spleen size measurements at 3 days poststroke (D), and platelet counts at 3 and 7 days poststroke (E). All data are presented as mean ± standard error of the mean. #P < .05 by one-way analysis of variance with Tukey’s post hoc analysis. ∗P < .05 and other specified P values by a t test (unpaired, 2-tailed). n = 5 to 14.