Figure 2.
IRF4 is a crucial target of HDAC1 in MM cells. (A-B) RPMI 8226, MM.1S, or U266 cells were transduced with IRF4 cDNA or Empty as a control by a retrovirus. Cells were further knocked down with HDAC1 or Luc shRNA. After puromycin selection, cell viability for 48 hours was assessed by the CCK-8 assay, and the whole cell lysates extracted were subjected to immunoblotting using the indicated antibodies. β-Actin served as a loading control. Relative expression levels of each target, which are normalized to its loading control, are shown below for each immunoblotting image. Error bars show the SD of triplicates. The cell growth rate in each cell line induced Empty or IRF4 cDNA with the shLuc set as 100% for control. ∗∗∗P < .001 significantly different from each cell induced with Empty cDNA with shHDAC1; the Tukey-Kramer multiple comparison test.

IRF4 is a crucial target of HDAC1 in MM cells. (A-B) RPMI 8226, MM.1S, or U266 cells were transduced with IRF4 cDNA or Empty as a control by a retrovirus. Cells were further knocked down with HDAC1 or Luc shRNA. After puromycin selection, cell viability for 48 hours was assessed by the CCK-8 assay, and the whole cell lysates extracted were subjected to immunoblotting using the indicated antibodies. β-Actin served as a loading control. Relative expression levels of each target, which are normalized to its loading control, are shown below for each immunoblotting image. Error bars show the SD of triplicates. The cell growth rate in each cell line induced Empty or IRF4 cDNA with the shLuc set as 100% for control. ∗∗∗P < .001 significantly different from each cell induced with Empty cDNA with shHDAC1; the Tukey-Kramer multiple comparison test.

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