USP9X expression in sporadic B-ALL samples does not differ between males and females with maintenance of normal patterns of X inactivation. (A) Expression of USP9X mRNA across 142 male (blue) and female (red) patients with B-ALL enrolled in phase 2 of TARGET. (B) No significant difference in expression levels of USP9X between males (n = 71) and females (n = 71) graphed as a violin plot. (C) Reads Per Kilobase of transcript per Million reads mapped (RPKM) of control gene XIST plotted against X chromosome copy number across patients with B-ALL (n = 127) on the left and RPKM of USP9X across patients with B-ALL (n = 123) on the right, with the regression line shown as a red dashed line.