The DLBCL TIME is heterogeneous. (A) Representative FOVs from 3 DLBCLs NOS and 1 reactive lymph node (rLN) demonstrating the heterogeneity of the TIME by hematoxylin and eosin staining (H&E) (top), MIBIscope pseudoimages colored per the indicated biomarkers (middle), and cell lineage maps colored per the indicated cell lineages (bottom). Bars represent ∼100 μm distance from 500 μm2 FOVs; digitized H&E visualized at an objective magnification ×4. (B) Percentage of lineage-assigned cells that are tumor cells or one of the indicated immune cell lineages from 51 DLBCLs and 8 rLN/tonsil controls colored as in panel A. Data are aggregated from 3 FOVs per case for most cases and a single FOV from controls. Cells without a defined cell lineage were classified as “other” and excluded. Data from individual FOVs are presented in supplemental Figure 2A.
