Figure 1.
HLA class I alleles affect the granzyme B loading in NK cells. (A) Granzyme B expression in KIR2DL1+ (blue), KIR2DL3+ (green), or KIR3DL1+ (orange) NK cells from donors with different HLA-C backgrounds (one representative experiment shown). (B) Granzyme B expression in KIR2DL3+ NK cells compared with the receptor-negative (Nil) NK cells from the same donor. C1/C1 donors (n = 143), C1/C2 donors (n = 147), and C2/C2 donors (n = 43). (C) Granzyme B expression in KIR2DL1+ NK cells compared with the Nil NK cells from the same C1/C1 donors (n = 140), C1/C2 donors (n = 128), and C2/C2 donors (n = 43). (D) Granzyme B expression in KIR3DL1+ NK cells compared with the Nil NK cells from the same Bw4- donors (n = 78) and Bw4+ donors (n = 170). (E) Granzyme B expression in KIR2DL1+ (blue), KIR2DL3+ (green), or KIR3DL1+ (orange) NK cells from donors with specific HLA alleles, HLA-C∗04 or HLA-C∗02; HLA-C∗03 or HLA-C∗07; Bw4-80T or Bw4-I80, respectively. (F) Granzyme B expression in KIR2DL3+ NK cells compared with the Nil NK cells from the same donor stratified by HLA-C1 alleles. C1/C1 donors (n = 140) and C2/C2 donors (n = 43). (G) Granzyme B expression in KIR2DL1+ NK cells compared with the Nil NK cells from the same donor stratified by HLA-C2 alleles. C1/C1 donors (n = 140) and C2/C2 donors (n = 43). (H) Granzyme B expression in KIR2DL3+ NK cells compared with the KIR2DL1+ NK cells from the same donor stratified by HLA-C1 and C2 alleles. C1/C2 donors (n = 125). (I) Granzyme B expression in KIR3DL1+ NK cells compared with the Nil NK cells from the same donor stratified by Bw4 alleles. Bw4 donors (n = 179). (B-D) Self-KIR NK cells are represented in red, and the nonself KIR NK cells are represented in blue. Each dot represents 1 donor. Whiskers show the fifth to 95th percentile. Bars show the median. One-way ANOVA tests followed by Kruskal-Wallis’ multiple comparison tests were performed in panels B, C and F-I. Mann-Whitney U test was performed in panel D. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; and ∗P < .05. ANOVA, analysis of variance; MFI, median fluorescence intensity.

HLA class I alleles affect the granzyme B loading in NK cells. (A) Granzyme B expression in KIR2DL1+ (blue), KIR2DL3+ (green), or KIR3DL1+ (orange) NK cells from donors with different HLA-C backgrounds (one representative experiment shown). (B) Granzyme B expression in KIR2DL3+ NK cells compared with the receptor-negative (Nil) NK cells from the same donor. C1/C1 donors (n = 143), C1/C2 donors (n = 147), and C2/C2 donors (n = 43). (C) Granzyme B expression in KIR2DL1+ NK cells compared with the Nil NK cells from the same C1/C1 donors (n = 140), C1/C2 donors (n = 128), and C2/C2 donors (n = 43). (D) Granzyme B expression in KIR3DL1+ NK cells compared with the Nil NK cells from the same Bw4- donors (n = 78) and Bw4+ donors (n = 170). (E) Granzyme B expression in KIR2DL1+ (blue), KIR2DL3+ (green), or KIR3DL1+ (orange) NK cells from donors with specific HLA alleles, HLA-C∗04 or HLA-C∗02; HLA-C∗03 or HLA-C∗07; Bw4-80T or Bw4-I80, respectively. (F) Granzyme B expression in KIR2DL3+ NK cells compared with the Nil NK cells from the same donor stratified by HLA-C1 alleles. C1/C1 donors (n = 140) and C2/C2 donors (n = 43). (G) Granzyme B expression in KIR2DL1+ NK cells compared with the Nil NK cells from the same donor stratified by HLA-C2 alleles. C1/C1 donors (n = 140) and C2/C2 donors (n = 43). (H) Granzyme B expression in KIR2DL3+ NK cells compared with the KIR2DL1+ NK cells from the same donor stratified by HLA-C1 and C2 alleles. C1/C2 donors (n = 125). (I) Granzyme B expression in KIR3DL1+ NK cells compared with the Nil NK cells from the same donor stratified by Bw4 alleles. Bw4 donors (n = 179). (B-D) Self-KIR NK cells are represented in red, and the nonself KIR NK cells are represented in blue. Each dot represents 1 donor. Whiskers show the fifth to 95th percentile. Bars show the median. One-way ANOVA tests followed by Kruskal-Wallis’ multiple comparison tests were performed in panels B, C and F-I. Mann-Whitney U test was performed in panel D. ∗∗∗∗P < .0001; ∗∗∗P < .001; ∗∗P < .01; and ∗P < .05. ANOVA, analysis of variance; MFI, median fluorescence intensity.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal