Figure 2.
Polytrauma induces inflammation, leading to oxidation and degradation of fibrinogen and causing coagulopathy in rats. Plasma was collected at the indicated time points from polytrauma-induced male rats (n = 11). (A) Cytokines were measured from plasma at the indicated time points by multiplex cytokine (IL-1α, IL-1β, IL-6, IL-10, IL-12, IL-17, IL-18, IL-33, GM-CSF, MCP-1, CXCL1, IFNγ, and TNFα) analysis kit (n = 11, mean ± SEM). ∗P < .05 vs 0 minute. ANOVA and Bonferroni post hoc test. (B) Fibrinogen, PT, and aPTT were measured using Ceveron alpha. ∗P < .05 vs 0 minute. ANOVA and Bonferroni post hoc test. (C) Relationships between IL-6 and fibrinogen, PT or aPTT, were determined by linear regression analysis using Pearson correlation coefficient. (D) Plasma was analyzed by OxyBlot and immunoblotting using fibrinogen Aα-, Bβ-, and γ-specific antibodies. Data are representative of 3 independent experiments. Ratio of a specific band intensity were measured using Image Studio and plotted on the right panel. ∗P < .05 vs 0 minute. ANOVA and Bonferroni post hoc test. ANOVA, analysis of variance; GM-CSF, granulocyte-macrophage colony-stimulating factor; INF, interferon; SEM, standard error of the mean.

Polytrauma induces inflammation, leading to oxidation and degradation of fibrinogen and causing coagulopathy in rats. Plasma was collected at the indicated time points from polytrauma-induced male rats (n = 11). (A) Cytokines were measured from plasma at the indicated time points by multiplex cytokine (IL-1α, IL-1β, IL-6, IL-10, IL-12, IL-17, IL-18, IL-33, GM-CSF, MCP-1, CXCL1, IFNγ, and TNFα) analysis kit (n = 11, mean ± SEM). ∗P < .05 vs 0 minute. ANOVA and Bonferroni post hoc test. (B) Fibrinogen, PT, and aPTT were measured using Ceveron alpha. ∗P < .05 vs 0 minute. ANOVA and Bonferroni post hoc test. (C) Relationships between IL-6 and fibrinogen, PT or aPTT, were determined by linear regression analysis using Pearson correlation coefficient. (D) Plasma was analyzed by OxyBlot and immunoblotting using fibrinogen Aα-, Bβ-, and γ-specific antibodies. Data are representative of 3 independent experiments. Ratio of a specific band intensity were measured using Image Studio and plotted on the right panel. ∗P < .05 vs 0 minute. ANOVA and Bonferroni post hoc test. ANOVA, analysis of variance; GM-CSF, granulocyte-macrophage colony-stimulating factor; INF, interferon; SEM, standard error of the mean.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal