NAC, Mito-Tempo, and AQB-565 alleviate the delay of thrombin and reptilase time and thinning of MCF from conditioned media at 3 and 4 hours in IL-6–stimulated leukocytes. IL-6–stimulated RBC-lysed leukocytes from healthy donors (n = 6) were incubated with fibrinogen, indicated various antioxidants and inhibitors as described in supplemental Methods; supplemental Figure 8B for indicated time periods. Conditioned medium was analyzed for thrombin and reptilase time (A, n = 6, mean ± SEM) and ROTEM as described in supplemental Methods (B, n = 4, mean ± SEM). ∗P < .001 vs control. ∗∗P < .001 vs IL-6. ANOVA and Bonferroni post hoc test. (B) Representative rotational thromboelastograms are overlaid with the conditioned media at 30 (green color), 180 (blue color), and 240 (red color) minutes. Quantitative results are plotted as MCF value on the right panel. ∗P < .001 vs control. ∗∗P < .001 vs IL-6. ANOVA and Bonferroni post hoc test. SEM, standard error of the mean; MCF, maximal clot firmness.