Maturation of platelet subpopulations. Characteristics of preterm neonates <32 weeks GA, 32 to 36 weeks GA, >36 weeks GA (t), infants 28d-2a, children 2 to 13a and adults >13a are displayed. Whole blood was preincubated with (A-C) TRAP-6 (5 μM), (D-F) ADP (5 μM), or (G-I) U46619 (2 μM). Automated clustering analysis was performed using FlowSOM. Representative curves are shown in panels A,D,G. Heat maps of clustering are shown in panels B,E, and H.