Figure 5.
Malignant T cells produce cytokines that induce alteration of FLG and FLG2 in the epidermis. (A) Supernatants obtained from MF1850, MF2059 or MF2000 cells, which had been cultured for 24 hours were analyzed for the cytokines IL-6, IL-13, IL-22, and OSM by enzyme-linked immunosorbent assay. Concentrations were given as picogram cytokine per milliliter supernatant. (B) NHEK were cultured for 2 hours in the presence or absence of receptor-blocking antibodies; IL-13Rα1 and dupilumab (IL-13), glycoprotein 130 (IL-6 and OSM), and IL22Rα1 (IL-22) to block cytokine signaling. NHEKs were then cultured for 24 hours with supernatants obtained from MF2059. The mRNA expression levels were analyzed by qPCR using β-actin as a reference gene. Gene expression was given as relative expressions. n = 3 for MF2059, MF1850, n = 1 for MF2000; ∗P < .05.

Malignant T cells produce cytokines that induce alteration of FLG and FLG2 in the epidermis. (A) Supernatants obtained from MF1850, MF2059 or MF2000 cells, which had been cultured for 24 hours were analyzed for the cytokines IL-6, IL-13, IL-22, and OSM by enzyme-linked immunosorbent assay. Concentrations were given as picogram cytokine per milliliter supernatant. (B) NHEK were cultured for 2 hours in the presence or absence of receptor-blocking antibodies; IL-13Rα1 and dupilumab (IL-13), glycoprotein 130 (IL-6 and OSM), and IL22Rα1 (IL-22) to block cytokine signaling. NHEKs were then cultured for 24 hours with supernatants obtained from MF2059. The mRNA expression levels were analyzed by qPCR using β-actin as a reference gene. Gene expression was given as relative expressions. n = 3 for MF2059, MF1850, n = 1 for MF2000; ∗P < .05.

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