Measuring beyond MRD 10−4in the CLL11 trial. (A) Comparison between MRD measurements obtained on samples from the CLL11 trial by ASO-qPCR or IGHV leader-based NGS. Each dot represents 1 measurement, and the color indicates the sampled anatomical compartment. The horizontal and vertical black lines indicate the limits of detection of the NGS assay and ASO-qPCR, respectively. Dots to the left of the vertical black lines signify MRD undetectability by ASO-qPCR, and dots below the horizontal dotted line signify MRD undetectability by leader-based NGS. The Pearson correlated coefficient was calculated using the log10-transformed values of samples in which MRD was quantifiable by both techniques. The black dotted line represents a perfect linear relationship. (B) Dot plot indicating MRD values obtained through IGHV leader-based NGS on samples in which ASO-qPCR was not feasible. Each dot represents 1 measurement, and the color indicates the sampled anatomical compartment. The horizontal black line indicates the limit of detection of the NGS assay. Dots below the horizontal dotted line signify MRD undetectability by leader-based NGS. BM, bone marrow; LoD, limit of detection; MRD+, detectable MRD; MRDu, undetectable MRD.
